|
Overview
 NanoSolution offers high-speed nanoscale proteomic analysis, the missing
link in post-genome research, using a direct nanoflow HPLC system with
the world's highest throughput. NanoSolution also provides proteomics consultation
services for large-scale analyses of protein expression patterns and protein
interactions. The NanoSolution direct nanoflow HPLC system has been installed
with high acclaim at a major Japanese pharmaceutical company and in research
institutes and national universities. As a member of the SNBL group (http://www.snbl.com/),
Japan's leading CRO, NanoSolution is ready to provide high-quality supplementary
services, and is currently in discussions with a number of pharmaceutical
companies and venture enterprises.
Technology
The NanoSolution approach to proteomics analysis is based on technology
invented by Professor Toshiaki Isobe of Tokyo Metropolitan University,
Professor Nobuhiro Takahashi of Tokyo University of Agriculture and Technology,
and Dr. Tohru Natsume, the Senior Research Scientist at the National Institute
of Advanced Industrial Science and Technology.
- Direct Nanoflow HPLC system
Ideal proteomics research conditions are now a reality. An online LC analysis
system equipped with a linear gradient pump (ReNCon) and an ultrafine column,
enables flow rates in nl/min.
This system has three distinct advantages:
*Microsamples
Existing methods require cultures of cells in liter units. However, with
this technique, analysis is possible using milliliter volumes prepared
from cells collected directly from the microtiter dish.
*Comprehensive Analysis
This technology makes possible the identification of membrane proteins,
glycoproteins, and low molecular weight proteins, none of which is achievable
with existing methods.
*High Throughput
Analyses that require months with existing technologies can now be completed
in a matter of days.
|
- 2DLC separation system
A multi-dimensional LC column connection enables the comprehensive identification
of over 1000 cellular proteins in a matter of days.
- Bioinformatics
Typical protein output data from HPLC and MS systems contains a large amount
of non-specific information, and the greatest challenge lies in the efficient
extraction of useful data. NanoSolution has developed proprietary software
that provides essential annotation for functional analysis, simplifying
the identification of specific proteins.
|
 |
| TOF-MS |
Service
- Proteome profiling
The first step in protein analysis is to identify target cells and comprehensively
examine and identify their protein content and kinetic profile. NanoSolution
can respond to client needs by providing high-precision and comprehensive
profiling of cell proteins using its proprietary direct nanoflow HPLC and
2DLC systems. Using this technology, identification of proteins (e.g.,
membrane proteins) that have not been found by conventional gel-based analysis
is possible. Thus, NanoSolution provides efficient methods for discovering
new target proteins.
- Interactomics
The examination of target protein-protein interactions accelerates the
collection of basic information for discovering new target proteins, and
for analyzing cellular signal transduction systems and functions. NanoSolution
can express target proteins in cells of interest, using cDNA provided by
the client. After pull-down, interacted proteins can be identified by direct
nanoflow HPLC. Data from some proteomics laboratories are confounded by
environmental contaminants such as dust, but NanoSolution offers uncontaminated
proteomics results derived in a clean booth from a know-how-based protocol.
President
Laboratory
- C/O Shin Nippon Biomedical Laboratories, Ltd.
Kainan Intelligence Park,
16-1 Minami-Akasaka, Kainan,
Wakayama 642-0017, Japan
Contact Address
- Toho Twintower Building 6F,
1-5-2 yuraku-cho, Chiyoda-ku,
Tokyo 100-0006, Japan
phone:+81-3-3593-8288 (Ken Takanashi / Hideyuki Hirama)
fax:+81-3-3500-5046
|
